Cold Spring Brook, Newton MA: eDNA Metabarcoding Results...

**A golden shiner from a tributary to Cold Spring Brook…**

The Massachusetts chapter of NFC has been working with Friends of Cold Spring Park and Newton Conservators to assess the condition of Cold Spring Brook within the park. Using technology provided by Jonah Ventures, NFC collected water samples from 4 sites in the watershed to be tested for eDNA metabarcoding. The results were quite interesting…

The eDNA samples were retested to try to resolve some issues with regard to what we felt were false positives. To be fair, eDNA metabarcoding is not, and doesn’t claim to be, as accurate as targeted species eDNA. The results were very similar in regard to fish, our primary focus…